📋 Workflow
Full workflow, products add-to-cart directly1
Sample prep
20 minHomogenize meat/egg/dairy samples and weigh
💡 Tip Keep samples cold for stability
⚠️ Caution Mix thoroughly before weighing
🧪 Materials for this step (click to shop by spec/brand)
2
Extraction
30 minExtract target drugs with acidified organic solvent or protein precipitant
💡 Tip Add internal standard to correct losses
⚠️ Caution Extract with thorough shaking
🧪 Materials for this step (click to shop by spec/brand)
3
SPE cleanup
40 minPurify the extract with HLB or cation-exchange SPE cartridge
💡 Tip Condition, load and elute
⚠️ Caution Do not dry out the bed
🧪 Materials for this step (click to shop by spec/brand)
4
LC-MS/MS measurement
60 minReconstitute, inject into LC-MS/MS and quantify multiple drugs by MRM
💡 Tip Matrix-matched calibration reduces interference
⚠️ Caution Confirm MRM transitions
🧪 Materials for this step (click to shop by spec/brand)
5
Quant & report
30 minCalculate residue by internal standard and compare to regulatory limits
💡 Tip Re-measure exceedances
⚠️ Caution Record concentration and units
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete protein precipitation interfering quantitation
- ② Low extraction recovery lowering results
- ③ Wrong SPE sorbent selection
- ④ Uncorrected matrix effect
- ⑤ Carryover causing false positives
❓ FAQ
+How many drugs at once?
Multi-residue LC-MS/MS can measure hundreds at once.
+Why SPE cleanup?
Remove fats/proteins, reduce matrix suppression.
+How to handle milk?
Protein precipitation then centrifuge the supernatant.