📋 Workflow
Full workflow, products add-to-cart directly1
Sample prep & weigh
20 minHomogenize the sample and weigh an appropriate amount
💡 Tip Homogenize whole produce with peel
⚠️ Caution Avoid cross-contamination
🧪 Materials for this step (click to shop by spec/brand)
2
QuEChERS extraction
30 minAdd extraction solvent and salts, vortex and centrifuge to achieve phase-partition extraction
💡 Tip Acetonitrile plus salt kit is universal
⚠️ Caution Vortex thoroughly
🧪 Materials for this step (click to shop by spec/brand)
3
Cleanup (d-SPE)
20 minPurify the supernatant with sorbents to remove pigments and lipids
💡 Tip PSA/C18 removes organic acids and fats
⚠️ Caution Optimize sorbent mass per matrix
🧪 Materials for this step (click to shop by spec/brand)
4
GC/LC-MS/MS measurement
60 minInject the extract and determine by tandem MS in MRM mode
💡 Tip Matrix-matched calibration compensates matrix effect
⚠️ Caution Use internal standards to correct recovery
🧪 Materials for this step (click to shop by spec/brand)
5
Quant & report
40 minCalculate residue by external/internal standard and compare to MRL
💡 Tip Convert result to original sample concentration
⚠️ Caution Re-measure to confirm MRL exceedance
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete extraction lowering residue
- ② Insufficient cleanup interfering quantitation
- ③ Uncorrected matrix effect biasing results
- ④ Standard matrix mismatching sample
- ⑤ Contamination/carryover causing false positives
❓ FAQ
+Single or multi-residue?
Multi-residue screening is more efficient with QuEChERS + LC-MS/MS.
+How to remove matrix effect?
Matrix-matched calibration or isotope internal standards.
+Which samples suit QuEChERS?
Produce, grain and other high-moisture matrices.