📋 Workflow
Full workflow, products add-to-cart directly1
Prepare components
30 minWeigh aldehyde, amine, isocyanide, etc., and fix the molar ratios.
💡 Tip Ugi/Passerini follow known stoichiometries
⚠️ Caution Bad ratios give side products
🧪 Materials for this step (click to shop by spec/brand)
2
Run the one-pot reaction
2–5 hAdd components sequentially or together, stir at set temperature to completion.
💡 Tip Activate at low T then warm for selectivity
⚠️ Caution Isocyanides are toxic/smelly; use a fume hood
🧪 Materials for this step (click to shop by spec/brand)
3
Monitor and workup
1 hConfirm product by LCMS/TLC, dilute, extract, dry, and concentrate.
💡 Tip Wash off excess components before isolation
⚠️ Caution MCRs give many by-products—don't skimp on purification
🧪 Materials for this step (click to shop by spec/brand)
4
Purify and identify
1–2 hPurify by column and confirm structure/purity by NMR/MS.
💡 Tip Multiple component by-products need careful column
⚠️ Caution Confirm structure before publishing
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong stoichiometry builds wrong scaffold
- ② High T triggers side reactions
- ③ Short isocyanide misses a fragment
- ④ Incomplete drying hydrolyzes at quench
- ⑤ Many by-products complicate column separation
❓ FAQ
+Many impurities one-pot?
Possible; purify carefully, but save steps.
+Common MCRs?
Ugi, Passerini, Biginelli, Hantzsch, etc.
+Good for libraries?
Excellent for building compound libraries quickly.