📋 Workflow
Full workflow, products add-to-cart directly1
Sample extraction
30 minExtract additives from food with solvent or buffer
💡 Tip Use ultrasonication to improve efficiency
⚠️ Caution Protect colorants from light degradation
🧪 Materials for this step (click to shop by spec/brand)
2
Cleanup & filtration
20 minCentrifuge or filter to remove matrix interference
💡 Tip Filter with 0.45 μm before injection
⚠️ Caution Prevent column clogging
🧪 Materials for this step (click to shop by spec/brand)
3
HPLC analysis
60 minInject the sample, separate on a C18 column and detect by UV
💡 Tip Choose isocratic or gradient elution as needed
⚠️ Caution Keep column temperature stable
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4
Calibration & quant
30 minCalculate additive content using an external calibration curve
💡 Tip Use multi-wavelength/DAD for confirmation
⚠️ Caution Linear range must cover sample concentration
🧪 Materials for this step (click to shop by spec/brand)
5
Report & assess
30 minCompare results to national limits and issue a report
💡 Tip Confirm exceedances by re-analysis
⚠️ Caution Keep original chromatograms
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete extraction lowering results
- ② Light degradation of colorants
- ③ Poor filtration introducing particulates
- ④ Standard matrix mismatching sample
- ⑤ Declining column performance reducing separation
❓ FAQ
+Preservatives & sweeteners together?
Yes, use a gradient HPLC method.
+Lipid- vs water-soluble extraction?
Choose solvent by polarity: aqueous or organic.
+Why high results?
Often co-eluting impurities interfering.