📋 Workflow
Full workflow, products add-to-cart directly1
Workspace and environment preparation
30 minTurn on biosafety cabinet, UV-sterilize and ventilate, wipe surfaces with 75% ethanol
💡 Tip UV-sterilize and ventilate beforehand
⚠️ Caution Arrange items to keep airflow clear
🧪 Materials for this step (click to shop by spec/brand)
2
Aseptic manipulation
1 hFlame bottle openings and tools, handle carefully to prevent contamination
💡 Tip Open containers toward sterile field
⚠️ Caution Wear gloves and spray alcohol throughout
🧪 Materials for this step (click to shop by spec/brand)
3
Contamination testing culture
1–2 daysInoculate sample or environmental swab onto nutrient medium, culture and observe colonies
💡 Tip Use various media to detect bacteria/fungi
⚠️ Caution Leave open plates as air-settle controls
🧪 Materials for this step (click to shop by spec/brand)
4
Read and disposition
30 minCheck for colony growth, assess sterility and document disposition
💡 Tip Regulate environmental monitoring
⚠️ Caution Positive samples need source investigation
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Items blocking airflow cause contamination
- ② Unflamed bottle openings
- ③ Crowded cabinet lowers protection
- ④ Contaminated plates give false positives
- ⑤ No environmental control confounds source
❓ FAQ
+Biosafety cabinet vs laminar flow?
Biosafety cabinet protects personnel with HEPA exhaust for bio-samples; flow hood protects samples.
+How to assess asepsis?
Monitoring via media, settle plates and negative controls periodically.
+When to change gloves?
Change after touching non-sterile surfaces, contamination or glove damage, then sanitize.