📋 Workflow
Full workflow, products add-to-cart directly1
Column conditioning
10 minPass organic solvent then water through the cartridge to wet and equilibrate the sorbent
💡 Tip Do not let the bed dry out
⚠️ Caution Load sample immediately after conditioning
🧪 Materials for this step (click to shop by spec/brand)
2
Sample loading
20 minPass the sample through the cartridge at a suitable flow rate so the target is retained
💡 Tip Adjust pH to favor retention
⚠️ Caution Control flow to avoid breakthrough
🧪 Materials for this step (click to shop by spec/brand)
3
Washing
10 minWash off interfering substances on the cartridge with a weaker solvent
💡 Tip Wash solvent should not be too strong
⚠️ Caution Avoid eluting the target during washing
🧪 Materials for this step (click to shop by spec/brand)
4
Elution & collect
15 minElute the target with a strong solvent and collect in a tube
💡 Tip Combine multiple elutions for higher recovery
⚠️ Caution Record elution volume for concentration calculation
🧪 Materials for this step (click to shop by spec/brand)
5
Evaporation & reconstitution
30 minConcentrate by nitrogen blowdown or rotary evaporation and reconstitute in injection solvent
💡 Tip Avoid over-drying that causes loss
⚠️ Caution Keep positive nitrogen flow
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Drying out the bed harming retention
- ② Fast loading causing breakthrough
- ③ Strong wash eluting the target
- ④ Insufficient elution lowering recovery
- ⑤ Over-concentration causing volatile loss
❓ FAQ
+How to choose SPE sorbent?
Match polarity: C18 RP, SAX/SCX ion exchange, etc.
+Low recovery?
Check pH, flow rate and elution strength.
+SPE for large water volumes?
Yes, use large cartridges to concentrate trace analytes.