📋 Workflow
Full workflow, products add-to-cart directly1
Electrode & meter setup
15 minMount the ISE and reference electrode and connect to the ion meter
💡 Tip Keep internal filling solution wet
⚠️ Caution Do not let the membrane dry
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2
Two-point calibration
20 minCalibrate the slope using two standards of known concentration
💡 Tip Standards should bracket expected sample concentration
⚠️ Caution Rinse the electrode before calibration
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3
Sample preparation
15 minPretreat the sample per protocol and adjust ionic strength
💡 Tip Add ionic-strength adjustment buffer
⚠️ Caution Avoid volatile loss
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4
Potential measurement
20 minImmerse the electrodes, wait for stable potential and record the reading
💡 Tip Stirring helps stabilize
⚠️ Caution Record after reading stabilizes
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5
Concentration & report
15 minCalculate ion concentration from the calibration or direct readout
💡 Tip Standard addition improves accuracy in complex samples
⚠️ Caution Convert to original sample concentration
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Dried membrane failing the electrode
- ② Inaccurate calibration biasing readings
- ③ Inconsistent ionic strength affecting activity
- ④ Slow response giving unstable readings
- ⑤ Clogged reference junction
❓ FAQ
+Why activity not concentration?
Potential responds to activity; adjuster maintains consistency.
+Slow electrode response?
Check membrane fouling/reference clog; clean and maintain.
+How often calibrate?
Two-point calibrate before each use.