📋 Workflow
Full workflow, products add-to-cart directly1
Sample collection and nucleic acid extraction
2 hCollect samples (swab/blood/tissue) and extract viral nucleic acid
💡 Tip Add internal controls
⚠️ Caution Preserve sample integrity in transit
🧪 Materials for this step (click to shop by spec/brand)
2
qRT-PCR amplification
2 hRun RT-qPCR with viral-specific primers/probes, quantify by Ct value
💡 Tip Include pos/neg controls and standard curve
⚠️ Caution Prevent aerosol contamination for false positives
🧪 Materials for this step (click to shop by spec/brand)
3
Antigen detection (optional)
2 hDetect viral protein antigen by ELISA or rapid test strip
💡 Tip Antigen is fast but lower sensitivity
⚠️ Caution Watch detection window and false negatives
🧪 Materials for this step (click to shop by spec/brand)
4
Result interpretation
30 minCall positive by Ct and/or antigen signal, verify validity with internal controls
💡 Tip Interpret with clinical/experimental context
⚠️ Caution Re-test when controls fail
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Aerosol contamination causes false positives
- ② Nucleic acid degradation gives false negatives
- ③ Impure extraction inhibits amplification
- ④ Antigen window short leads to misses
- ⑤ Failed internal control invalidates results
❓ FAQ
+PCR vs antigen detection?
PCR sensitive, early, quantitative; antigen fast but less sensitive.
+How to prevent false positives?
Separate rooms, prevent aerosol, use dUTP/UNG and strict controls.
+What does higher Ct mean?
Higher Ct means lower viral load; above threshold is negative.