📋 Workflow
Full workflow, products add-to-cart directly1
Wavelength & baseline
15 minWarm up the instrument, zero with blank and scan a baseline over the full range
💡 Tip Warm up 20-30 min to stabilize the lamp
⚠️ Caution Use quartz cuvettes in the UV range
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2
Standard solution prep
20 minPrepare a series of standard solutions and measure their absorbance
💡 Tip Keep absorbance within 0.1-1.0
⚠️ Caution Choose the maximum absorption wavelength λmax
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3
Sample measurement
15 minDilute the sample to a suitable range and measure absorbance
💡 Tip Sample must be clear without interfering peaks
⚠️ Caution Filter turbid samples first
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4
Calibration & quant
15 minPlot absorbance vs concentration to fit a calibration curve and derive sample concentration
💡 Tip Target R² ≥ 0.999
⚠️ Caution Dilute if above the linear range
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Dirty cuvette causing reading bias
- ② Wrong wavelength lowering sensitivity
- ③ Absorbance above linear range
- ④ Blank inconsistent with sample
- ⑤ No warm-up causing baseline drift
❓ FAQ
+What absorbance is best?
0.1–1.0 gives minimal error.
+How to measure colored samples?
Use its specific absorption wavelength or dilute.
+Can glass cuvettes be used?
Use quartz for UV; glass is fine for visible.