📋 Workflow
Full workflow, products add-to-cart directly1
Matrix/feeder preparation
1 hCoat dishes (laminin/Matrigel) or seed feeder cells to support stem cells
💡 Tip Matrix concentration and uniform coating matter
⚠️ Caution Allow matrix to coat fully
🧪 Materials for this step (click to shop by spec/brand)
2
Medium and seeding
30 minResuspend and seed cells in serum-free medium with factors like LIF
💡 Tip Seeding density affects colony formation
⚠️ Caution Fresh medium to avoid factor degradation
🧪 Materials for this step (click to shop by spec/brand)
3
Maintenance and feeding
30 min dailyFeed fresh medium daily, maintain appropriate confluency and undifferentiated morphology
💡 Tip Monitor morphology to prevent spontaneous differentiation
⚠️ Caution Avoid over-confluency causing differentiation
🧪 Materials for this step (click to shop by spec/brand)
4
Passaging and cryopreservation
1 hPassage with gentle enzyme or EDTA, cryopreserve extra cells
💡 Tip Gentle passaging preserves potency
⚠️ Caution Over-digestion damages pluripotent cells
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong medium/factors cause spontaneous differentiation
- ② Over-passaging causes karyotype abnormality or differentiation
- ③ Over-digestion damages viability
- ④ Over-confluency promotes differentiation
- ⑤ Contamination hard to eliminate and skews results
❓ FAQ
+How to confirm undifferentiated state?
Check compact colony morphology and pluripotency markers like Nanog, Oct4.
+Why daily feeding?
Maintain factor levels and clear differentiation-promoting metabolites, preventing spontaneous differentiation.
+Feeder vs feeder-free?
Feeder-free with matrix/factors is more controlled and standardized; feeder is classic but batch-variable.