📋 Workflow
Full workflow, products add-to-cart directly1
Single-cell suspension preparation
1 hGently dissociate tissue/culture into high-viability single-cell suspension
💡 Tip Viability and clump control are critical
⚠️ Caution Avoid over-dissociation causing damage/transcriptional response
🧪 Materials for this step (click to shop by spec/brand)
2
Droplet partitioning and barcoding
1 hUse microfluidics to partition single cells with barcoded gel beads into droplets
💡 Tip Target several thousand UMIs per cell
⚠️ Caution Control cell:bead ratio by concentration
🧪 Materials for this step (click to shop by spec/brand)
3
Library construction and sequencing
1–3 daysAmplify barcoded cDNA with adapters, build library and sequence
💡 Tip Depth adjusted per cell number
⚠️ Caution Prevent barcode cross-sample contamination
🧪 Materials for this step (click to shop by spec/brand)
4
Dimensionality reduction and clustering
1 dayQC, quantify, deconvolute cells by barcode, reduce dimensionality and cluster to identify cell types
💡 Tip Annotate clusters with marker genes
⚠️ Caution Remove doublets and low-quality cells
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Low viability degrades data quality
- ② Doublets/empty droplets disturb analysis
- ③ Dissociation-induced transcriptome changes
- ④ Low depth misses low-expression
- ⑤ Batch effects confound cell identity
❓ FAQ
+Single-cell vs bulk RNA-seq?
Single-cell resolves per-cell expression and heterogeneity; bulk gives population average.
+How to identify cell types?
Annotate clusters with known marker genes and reference atlases.
+How to remove doublets?
Use software methods based on UMI/gene counts to predict and filter doublets.