📋 Workflow
Full workflow, products add-to-cart directly1
Form diastereomeric salt/derivative
1–2 hReact the racemic acid/base with a chiral resolving agent to form separable diastereomeric salts.
💡 Tip Resolve with a suitably configured chiral base/acid
⚠️ Caution Sub-stoichiometric chiral agent can improve yield
🧪 Materials for this step (click to shop by spec/brand)
2
Fractional crystallization
2–4 hUse solubility differences to fractionally crystallize/precipitate one-preferred salt.
💡 Tip Slow cooling helps crystal-pure separation
⚠️ Caution May need several cycles for optical purity
🧪 Materials for this step (click to shop by spec/brand)
3
Recover single enantiomer
1–2 hLiberate the desired enantiomer from the resolved salt by acid/base treatment and extraction.
💡 Tip Recover the resolving agent for reuse
⚠️ Caution Avoid damaging sensitive groups during acid/base
🧪 Materials for this step (click to shop by spec/brand)
4
Determine enantiopurity
40 minDetermine ee and configuration by chiral HPLC/GC or optical rotation.
💡 Tip Chiral column quantitation is most direct
⚠️ Caution Relate rotation to standard/literature
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong resolving agent gives poor separation
- ② Rushed crystallization misses optical purity
- ③ Harsh acid/base damages while recovering
- ④ Not recycling resolving agent raises cost
- ⑤ Rotation-alone ee has large error
❓ FAQ
+Max theoretical yield?
~50% of one enantiomer; repeat cycles for purity.
+Choose resolving agent?
Pick a common chiral acid/base forming crystallizable salts.
+Resolution vs asymmetric catalysis?
Use resolution when the racemate is easy and catalysis is hard.