📋 Workflow
Full workflow, products add-to-cart directly1
Develop the analytical method
1 hUse an analytical column to fix mobile phase, gradient, and resolution.
💡 Tip Scale to prep only when R>1.5
⚠️ Caution Direct scale-up of small-column results overloads
🧪 Materials for this step (click to shop by spec/brand)
2
Prepare and dissolve sample
20 minDissolve the sample in a minimum of starting mobile phase; filter if needed.
💡 Tip Dissolving in starting mobile phase sharpens peaks
⚠️ Caution Large particles block the prep column
🧪 Materials for this step (click to shop by spec/brand)
3
Preparative injection and separation
1–3 hInject onto the prep column, run the developed method, collect the target peak by detector trigger.
💡 Tip Use DAD or MS to guide peak cutting
⚠️ Caution Overloading overlaps peaks
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4
Collect and concentrate fractions
40 minCollect fractions by auto-collector, pool same-peak fractions, evaporate solvent.
💡 Tip Evaporate under vacuum to protect sensitive product
⚠️ Caution Over-vigorous evaporation loses sample
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5
Verify purity
20 minRe-check purity on analytical HPLC; confirm by LC-MS if needed.
💡 Tip >95% is usually ready for downstream
⚠️ Caution UV-invisible impurities may still exist
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Not scaling flow with column diameter
- ② Overload overlaps peaks
- ③ Cutting too wide pools impurity peaks
- ④ Unfiltered sample blocks the column
- ⑤ Solvent evaporation loses sample
❓ FAQ
+Yield vs resolution trade-off?
Increase load when R>1.5; otherwise reduce it.
+How to scale a small-column method?
Scale flow and load by the square of diameter ratio.
+Collected impurity?
Re-run that peak region or tweak the gradient.