📋 Workflow
Full workflow, products add-to-cart directly1
Resin and first amino acid
1 hChoose suitable solid resin and pre-load the first amino acid or linker
💡 Tip Resin loading/type determines product
⚠️ Caution Swell resin thoroughly
🧪 Materials for this step (click to shop by spec/brand)
2
Deprotection and coupling cycles
HoursRepeat Fmoc deprotection and activated amino acid coupling to extend from C- to N-terminus
💡 Tip Optimize coupling reagent equivalents/time
⚠️ Caution Monitor completeness to avoid deletions
🧪 Materials for this step (click to shop by spec/brand)
3
Cleavage and deprotection
2–4 hCleave peptide from resin and remove side-chain protecting groups with cleavage cocktail
💡 Tip Use scavengers to suppress side reactions
⚠️ Caution Prepare cleavage cocktail fresh
🧪 Materials for this step (click to shop by spec/brand)
4
Purification and QC
HoursPurify peptide by reverse-phase HPLC, verify by mass spec and purity analysis
💡 Tip Purity meets downstream application needs
⚠️ Caution Lyophilize and seal to avoid moisture
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete coupling causes deletion sequences
- ② Poor resin swelling uneven reaction
- ③ Side reactions (racemization/aggregation) lower yield
- ④ Cleavage byproducts contaminate
- ⑤ Long or poorly soluble peptides hard to synthesize
❓ FAQ
+How long can peptides be?
Routine solid-phase usually 10-50 residues; longer needs complex strategies.
+How to improve purity?
Optimize coupling/cleavage, monitor reaction, purify by RP-HPLC and re-qualify.
+How to confirm structure?
Confirm molecular weight by MS vs theoretical, optionally sequence or amino acid analysis.