📋 Workflow
Full workflow, products add-to-cart directly1
Cell and electrode preparation
30 minPrepare adherent or acutely-isolated cells; pull and back-fill glass electrodes with internal solution
💡 Tip Tip resistance (2-5 MΩ) is critical
⚠️ Caution Avoid air bubbles in electrode
🧪 Materials for this step (click to shop by spec/brand)
2
Form seal
30 minAdvance electrode to cell under microscope, apply suction to form giga-ohm seal
💡 Tip Positive pressure prevents clogging
⚠️ Caution Advance gently to avoid damaging cell
🧪 Materials for this step (click to shop by spec/brand)
3
Break-in or configuration
10 minBreak membrane by suction/voltage pulse for whole-cell, or excise inside-out/outside-out patches
💡 Tip Choose configuration per question
⚠️ Caution Excessive break-in damages cell
🧪 Materials for this step (click to shop by spec/brand)
4
Record and analyze
1–3 hApply voltage/current protocols to record current/potential, analyze amplitude and channel kinetics by software
💡 Tip Series resistance compensation improves accuracy
⚠️ Caution Correct drift and junction potential
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Seal failure or insufficient resistance
- ② Electrode or tip clogging
- ③ High series resistance distorts recording
- ④ Uncorrected junction potential offsets
- ⑤ Vibration/EM interference adds noise
❓ FAQ
+Whole-cell vs single-channel?
Whole-cell records whole-cell current; excised patch records single-channel activity.
+How to reduce noise?
Use Faraday cage, vibration isolation, optimize electrode and grounding.
+Why series resistance compensation?
Series resistance causes voltage error and current distortion; compensation improves accuracy.