📋 Workflow
Full workflow, products add-to-cart directly1
Matrix embedding
1 hMix stem cells with Matrigel, drop into wells to gel as 3D environment
💡 Tip Keep Matrigel on ice to prevent gelling
⚠️ Caution Disperse cells evenly
🧪 Materials for this step (click to shop by spec/brand)
2
Organoid medium
30 minAdd organoid medium containing factors like Wnt, R-spondin, Noggin
💡 Tip Customize factor cocktail per tissue type
⚠️ Caution Prepare fresh to preserve factor activity
🧪 Materials for this step (click to shop by spec/brand)
3
Long-term culture and passaging
2–6 weeksFeed regularly, when organoids reach size, dissociate mechanically or enzymatically, re-embed
💡 Tip Passaging preserves structure and stemness
⚠️ Caution Over-passaging causes differentiation
🧪 Materials for this step (click to shop by spec/brand)
4
Validation and functional analysis
1–2 daysVerify structure and function by immunofluorescence, histology and functional assays
💡 Tip Confirm differentiation with multi-lineage markers
⚠️ Caution Watch for tumorigenic/aberrant growth
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Matrigel batch variability affects formation
- ② Wrong factor cocktail causes aberrant differentiation
- ③ Over-passaging loses structure/stemness
- ④ Central necrosis/hypoxia restricts growth
- ⑤ Contamination or variability causes failure
❓ FAQ
+How to maintain organoids long-term?
Regular feeding, timely passaging and re-embedding, maintain factor cocktail and low-attachment.
+Why Matrigel?
Provides basement-membrane-like ECM supporting 3D self-organization and polarity.
+Can organoids be used for drug screening?
Yes, organoids retain patient/tissue specificity, suitable for efficacy and personalized assessment.