📋 Workflow
Full workflow, products add-to-cart directly1
Design modification strategy
1 hSelect modification site and type (terminal, internal, specific group) per application
💡 Tip Position affects function/stability
⚠️ Caution Balance modification level with activity
🧪 Materials for this step (click to shop by spec/brand)
2
Select modification monomers
30 minSelect phosphorothioate, 2'-OMe/LNA, fluorescent modified phosphoramidite monomers
💡 Tip Monomer purity determines quality
⚠️ Caution Handle monomers dry
🧪 Materials for this step (click to shop by spec/brand)
3
Synthesis and modification introduction
Hours-1 dayIncorporate modified monomers during oligo synthesis or perform site-specific conjugation
💡 Tip Set positions in synthesis cycle
⚠️ Caution Monitor coupling efficiency
🧪 Materials for this step (click to shop by spec/brand)
4
Purification and performance verification
1 dayPurify modified product, verify modification site/structure, test stability/activity
💡 Tip MS confirms modification present
⚠️ Caution Modification may affect annealing/hybridization
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Excess modification impairs hybridization/activity
- ② Wrong position compromises function
- ③ Impure monomers cause defects
- ④ Modification complicates purification/QC
- ⑤ Incomplete conjugation loses label
❓ FAQ
+What is phosphorothioate for?
Increases nuclease resistance and in vivo stability, common in antisense/siRNA.
+How to confirm modification?
Confirm mass shift and modified-site fragments by mass spec.
+Modification affects hybridization?
Choose low-impact modifications like LNA (increases affinity) and optimize sequence.