📋 Workflow
Full workflow, products add-to-cart directly1
Sample lysis
15 minAdd lysis buffer to disrupt cells/tissue, releasing nucleic acids and inactivating nucleases
💡 Tip RNA extraction needs RNase-free conditions
⚠️ Caution Grind frozen tissue thoroughly
🧪 Materials for this step (click to shop by spec/brand)
2
Bind nucleic acid
10 minAdjust with isopropanol, load onto spin column or magnetic beads to bind nucleic acid
💡 Tip High salt promotes silica binding
⚠️ Caution Buffers differ for RNA vs DNA
🧪 Materials for this step (click to shop by spec/brand)
3
Washing
10 minWash several times with ethanol-containing wash buffer to remove proteins, salts and impurities
💡 Tip Wash thoroughly but avoid over-drying
⚠️ Caution Over-drying hampers elution
🧪 Materials for this step (click to shop by spec/brand)
4
Elution and QC
15 minElute with nuclease-free water or elution buffer, measure OD260/280 and concentration for purity
💡 Tip A260/280 ~2.0 for RNA, ~1.8 for DNA
⚠️ Caution Check integrity (RNA gel/amplicon)
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① RNA degraded by RNase causing poor integrity
- ② DNA contaminated with protein/salt
- ③ Small elution or over-drying lowers yield
- ④ Insufficient grinding gives incomplete extraction
- ⑤ Residual ethanol inhibits downstream enzymatic reactions
❓ FAQ
+Can RNA and DNA be extracted together?
Extract total nucleic acids then partition, or use dual-purification kits.
+High A260/280 ratio?
High ratio often from residual phenol/ethanol, needs re-purification.
+How to avoid RNA degradation?
Work on ice, use RNase-free consumables, include RNase inhibitors, store at -80°C.