📋 Workflow
Full workflow, products add-to-cart directly1
Dissolve the sample
10 minDissolve 5–15 mg in 0.5 mL deuterated solvent, transfer to an NMR tube.
💡 Tip Clear, particle-free solutions give cleaner spectra
⚠️ Caution Deuterated solvents absorb H₂O; keep sealed
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2
Load and shim
10 minLoad the tube, lock and shim until peaks are symmetric and sharp.
💡 Tip Good shimming narrows lines and improves integration
⚠️ Caution Gas bubbles disturb shimming
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3
Acquire spectra
15–30 minSet the ¹H pulse sequence, scans, and spectral width, acquire; then ¹³C and needed 2D.
💡 Tip Run DEPT/HSQC when needed for assignment
⚠️ Caution Too few scans give poor ¹³C S/N
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4
Analyze and assign
20 minProcess in software, integrate and assign peaks, compare with the expected structure.
💡 Tip Multiplicities and J values reveal substitution patterns
⚠️ Caution Don't mistake residual solvent peaks for product
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⚠️ 5 Common Beginner Mistakes
- ① Too little sample, ¹³C shows nothing
- ② Water/solvent peak obscures target signal
- ③ Misassigning residual solvent as product
- ④ Poor shimming broadens lines and skews integration
- ⑤ Paramagnetic impurity distorts peaks
❓ FAQ
+Which deuterated solvent?
CDCl₃ for routine; DMSO-d₆ or D₂O for polar samples.
+Assess purity by NMR?
Check for unexpected extra peaks and integral ratios.
+When to run 2D?
When assignment is ambiguous or overlapping—COSY/HSQC/HMBC.