📋 Workflow
Full workflow, products add-to-cart directly1
Brain tissue harvest
30 minEuthanize animal, aseptically remove brain, isolate target region (cortex/hippocampus) on ice
💡 Tip Rapid cold harvest preserves viability
⚠️ Caution Sterile operation prevents contamination
🧪 Materials for this step (click to shop by spec/brand)
2
Digestion and dissociation
30 minDigest with papain or trypsin, gently triturate to single cells
💡 Tip Gentle trituration protects processes
⚠️ Caution Avoid mechanical damage and over-digestion
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3
Seeding and matrix
1 hCoat dishes with poly-L-lysine/laminin, seed neurons at suitable density
💡 Tip Coating aids adhesion and outgrowth
⚠️ Caution Density affects network formation
🧪 Materials for this step (click to shop by spec/brand)
4
Long-term maintenance
1–3 weeksCulture in neuronal medium with trophic factors, feed regularly and control glial ratio
💡 Tip Use cytarabine to limit glial overgrowth
⚠️ Caution Handle mature neurons gently
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Slow/warm harvest lowers viability
- ② Over-digestion damages neurons
- ③ Harsh trituration breaks processes
- ④ Glial overgrowth covers neurons
- ⑤ Contamination kills neurons
❓ FAQ
+How to control glial ratio?
Use mitotic inhibitors like cytarabine or ratio-based co-culture.
+How long to mature?
Most primary neurons form mature networks in 2-3 weeks; timing varies by region.
+Why matrix coating?
Provides adhesion and trophic signals for attachment, outgrowth and networking.