📋 Workflow
Full workflow, products add-to-cart directly1
Tune & calibration
30 minTune the MS and calibrate the mass axis with calibration solution
💡 Tip Periodically verify mass accuracy
⚠️ Caution Keep the ion source clean
🧪 Materials for this step (click to shop by spec/brand)
2
Sample prep
30 minDilute the sample to a suitable concentration in the mobile phase/solvent
💡 Tip Remove salts/lipids to avoid ion suppression
⚠️ Caution Use internal standard to correct ion suppression
🧪 Materials for this step (click to shop by spec/brand)
3
MS method development
30 minSet ionization mode, source temperature and monitored ion transitions
💡 Tip MRM improves selectivity
⚠️ Caution Avoid source temperature that causes in-source decomposition
🧪 Materials for this step (click to shop by spec/brand)
4
Injection & acquisition
40 minInject blank, standards and samples; acquire mass spectral data
💡 Tip Gradient elution improves peak shape
⚠️ Caution Avoid carryover contamination
🧪 Materials for this step (click to shop by spec/brand)
5
Data & quant
30 minIntegrate peaks, quantify by internal standard and report
💡 Tip Isotope internal standard is most accurate
⚠️ Caution Confirm no interference on MRM transitions
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Ion suppression lowering results
- ② Matrix contamination lowering sensitivity
- ③ Mass axis drift affecting accuracy
- ④ Carryover causing false positives
- ⑤ Poor cleaning contaminating the source
❓ FAQ
+Why internal standard?
Corrects ion suppression and prep losses.
+ESI or APCI?
ESI for polar/bio molecules; APCI for non-polar small molecules.
+MRM vs full scan?
MRM has better selectivity, lower noise, higher sensitivity.