📋 Workflow
Full workflow, products add-to-cart directly1
Medium preparation
1 hPrepare agar or broth medium, autoclave and pour plates or aliquot
💡 Tip Choose medium per microbe type
⚠️ Caution Sterilize thoroughly to avoid contamination
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2
Inoculation and culture
1–2 daysInoculate colonies with loop, culture at suitable temperature (e.g. 37°C) and oxygen condition
💡 Tip Anaerobes need anaerobic jar/chamber
⚠️ Caution Sterile technique prevents cross-contamination
🧪 Materials for this step (click to shop by spec/brand)
3
Growth monitoring and purification
1 hMonitor OD or count, streak plates to purify single colonies
💡 Tip Single colonies confirm purity
⚠️ Caution Full streaking yields isolated colonies
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4
Stock preservation
30 minAdd glycerol to make cryostock, store at -80°C for long-term preservation
💡 Tip Glycerol final ~15-25%
⚠️ Caution Label strain and date
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong medium/pH for target microbe
- ② Poor aseptic technique causes contamination
- ③ Anaerobes exposed to oxygen fail to grow
- ④ Wrong temperature/time
- ⑤ Missing label causes strain confusion
❓ FAQ
+How to confirm pure culture?
Isolate uniform single colonies; verify by Gram stain or 16S sequencing.
+Why match oxygen conditions?
Aerobes, facultative and anaerobes differ in oxygen tolerance and supply needs.
+How to preserve long-term?
Store in glycerol at -80°C or lyophilize; avoid repeated passaging to prevent mutations.