📋 Workflow
Full workflow, products add-to-cart directly1
Inoculum preparation
30 minPick colonies, adjust to McFarland standard (~1.5×10^8 CFU/mL) then dilute
💡 Tip Inoculum concentration affects results
⚠️ Caution Use fresh log-phase culture
🧪 Materials for this step (click to shop by spec/brand)
2
Drug gradient preparation
30 minSerial-dilute antibiotic into a series of concentrations directly in 96-well plate
💡 Tip Range should cover expected MIC
⚠️ Caution Consistent technique for reproducibility
🧪 Materials for this step (click to shop by spec/brand)
3
Inoculate and incubate
16–24 hAdd diluted bacteria to ~5×10^5 CFU/mL final, incubate at suitable temperature
💡 Tip Include growth (no drug) and negative controls
⚠️ Caution Growth control verifies viability
🧪 Materials for this step (click to shop by spec/brand)
4
Read MIC
15 minVisually assess turbidity, read lowest drug concentration without visible growth
💡 Tip Read per CLSI guidelines
⚠️ Caution Contamination or faint growth affects reading
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Non-standard inoculum skews MIC
- ② Dilution error accumulates
- ③ Medium/incubation not matching CLSI
- ④ No control hampers reading
- ⑤ Contamination gives false resistance/susceptibility
❓ FAQ
+MIC vs MBC?
MIC inhibits growth; MBC kills 99.9% of bacteria.
+How to interpret R/S?
Compare MIC to CLSI breakpoints: below susceptible threshold=S, above=resistant.
+Why growth control?
Confirms inoculum viability so no-drug wells grow, validating the assay.