📋 Workflow
Full workflow, products add-to-cart directly1
Solvent selection
15 minChoose an organic solvent immiscible with water with good affinity for the target
💡 Tip Select solvent by analyte polarity
⚠️ Caution Mind solvent toxicity and volatility
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2
Add & shake
20 minAdd aqueous sample and extraction solvent to the funnel and shake thoroughly
💡 Tip Periodically vent to release pressure
⚠️ Caution Let it settle to separate layers
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3
Layer separation
20 minAfter layering, carefully drain the lower or upper organic phase
💡 Tip Repeat extraction to raise recovery
⚠️ Caution Identify which layer is which
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4
Dry & concentrate
30 minDry the organic phase with anhydrous sodium sulfate and concentrate
💡 Tip Filter off the drying agent before concentration
⚠️ Caution Avoid high concentration temperature
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5
Reconstitute & inject
20 minReconstitute the concentrate in injection solvent for analysis
💡 Tip Mix well after making up to volume
⚠️ Caution Record volume and dilution factor
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Too few extractions lowering recovery
- ② Insufficient settling causing cloudy layers
- ③ Improper venting spraying out liquid
- ④ Collecting wrong layer
- ⑤ Over-concentration losing sample
❓ FAQ
+How to judge clean layering?
Settle until the interface is clear and sharp.
+How many extractions?
Usually 2–3 times suffices for high recovery.
+Why dry?
Remove water that could interfere with analysis.