📋 Workflow
Full workflow, products add-to-cart directly1
Cell and virus preparation
30 minCulture cells to appropriate confluency, thaw virus and calculate required MOI
💡 Tip Set MOI per vector/cell type
⚠️ Caution Avoid repeated freeze-thaw of virus
🧪 Materials for this step (click to shop by spec/brand)
2
Viral transduction
4–24 hAdd virus to medium at MOI, optionally with enhancer (polybrene) to boost efficiency
💡 Tip Low MOI reduces toxicity
⚠️ Caution Handle infectious virus in biosafety cabinet
🧪 Materials for this step (click to shop by spec/brand)
3
Medium change and detection
1–2 daysReplace medium to remove virus, culture to expression time, detect expression or infection efficiency
💡 Tip GFP marker aids efficiency check
⚠️ Caution Time depends on expression kinetics
🧪 Materials for this step (click to shop by spec/brand)
4
Efficiency confirmation and follow-up
1 hAssess transduction/infection efficiency and choose suitable MOI for downstream experiments
💡 Tip Quantify efficiency by FACS
⚠️ Caution Prevent viral spread and contamination
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① High MOI causes cytotoxicity
- ② Thawed virus loses activity
- ③ Low transduction efficiency
- ④ Repeated freeze-thaw fails virus batches
- ⑤ Non-standard dose skews infection models
❓ FAQ
+What is MOI?
Multiplicity of infection: viral particles/infectious units per cell determining infection level.
+How to improve transduction?
Add polybrene, longer exposure, higher virus concentration, or higher-tropism serotype.
+When to check expression after infection?
Vector-dependent: lentivirus 24-72h, AAV longer; determine empirically.