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IC

Ion separation & quant
🎯 Difficulty ★★☆ Medium Duration About 2–3 h 🎯 Use Analysis of inorganic anions/cations and organic acids in water and food
📖 Principle
Ions separate on an ion-exchange column by affinity difference and are quantified by conductivity
Below are 5 steps. Open each to see how to do it and add products to your shared list.

📋 Workflow

Full workflow, products add-to-cart directly
1

Eluent prep

20 min
Prepare carbonate/hydroxide eluent and degas
💡 Tip Hydroxide systems need online generation
⚠️ Caution Use ultrapure water to avoid ion contamination
🧪 Materials for this step (click to shop by spec/brand)
2

Column install & equilibrate

30 min
Install guard and analytical columns; equilibrate with eluent until baseline is stable
💡 Tip Equilibrate for 20-30 min
⚠️ Caution Avoid eluent flow interruption
🧪 Materials for this step (click to shop by spec/brand)
3

Standard & sample prep

30 min
Prepare anion/cation standard series; dilute and filter samples before injection
💡 Tip Dilute high-concentration samples first
⚠️ Caution Filter samples through 0.22 μm
🧪 Materials for this step (click to shop by spec/brand)
4

Injection & detection

30 min
Inject sample through suppressor and quantify with conductivity detector
💡 Tip Suppressor lowers background for higher sensitivity
⚠️ Caution Keep suppressor wet; avoid bubbles
🧪 Materials for this step (click to shop by spec/brand)
5

Data & report

30 min
Integrate peaks, quantify via calibration curve and issue a report
💡 Tip Quantify multiple ions simultaneously
⚠️ Caution Verify retention times and ion identity
🧪 Materials for this step (click to shop by spec/brand)

⚠️ 5 Common Beginner Mistakes

  • Impure water introducing ion interferences
  • Unfiltered samples clogging the column
  • Eluent concentration drift affecting retention time
  • Failed suppressor preventing detection
  • High-concentration residue contaminating the system

❓ FAQ

+Which ions can be detected?
Common inorganic anions/cations and some organic acids.
+Why unstable baseline?
Often eluent/suppressor issues or air in lines.
+Can anions/cations run together?
Usually need separate anion and cation columns.

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Data authenticity: Product categories from JW Supply Chain standard taxonomy; method→step→product connected. Last updated: 2026-08-23 · v3.0