📋 Workflow
Full workflow, products add-to-cart directly1
Assay development
1 dayDevelop biochemical/cellular assay, optimize substrate, enzyme/cell amount and incubation
💡 Tip Assay needs large, stable window
⚠️ Caution Measure Z-factor for robustness
🧪 Materials for this step (click to shop by spec/brand)
2
Compound plate preparation
1 hTransfer compounds from library to assay plates, set concentrations and controls
💡 Tip Use high/low controls for normalization
⚠️ Caution Keep DMSO concentration consistent
🧪 Materials for this step (click to shop by spec/brand)
3
HTS run
1–2 daysAutomate dispensing, incubate and read whole plates
💡 Tip Automation reduces batch variation
⚠️ Caution Maintain across-plate consistency
🧪 Materials for this step (click to shop by spec/brand)
4
Data analysis and hits
1 dayNormalize data, compute % inhibition, call hits by statistical threshold and revalidate
💡 Tip Control hit rate and false positives
⚠️ Caution Require replication and dose confirmation
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Small assay window hampers screening
- ② Poor Z-factor makes results unreliable
- ③ Liquid handling error across plates
- ④ High false positives waste validation
- ⑤ Hits not confirmed by replication
❓ FAQ
+What is Z-factor?
Measures assay window vs variation; >0.5 is suitable for screening.
+How to control false positives?
Replicate, dose-response confirmation, controls to exclude non-specific/physical interference.
+Biochemical vs cellular screening?
Biochemical is target-specific and high-throughput; cellular is more physiological but affected by toxicity.