📋 Workflow
Full workflow, products add-to-cart directly1
Prepare sample
30 minDissolve the polymer in the mobile phase (e.g., THF) and filter.
💡 Tip Full dissolution avoids artifacts
⚠️ Caution THF is flammable; ventilate
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2
Prepare standards
30 minPrepare a series of known-molecular-weight polystyrene standards.
💡 Tip Standards should bracket the sample range
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3
Equilibrate & inject
30 minEquilibrate at a set flow, then inject standards and samples in turn.
💡 Tip Constant flow keeps results comparable
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4
Data analysis
30 minBuild a calibration curve and compute Mw, Mn, and dispersity.
💡 Tip Note the standard system for relative MW
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⚠️ 5 Common Beginner Mistakes
- ① Poor dissolution distorts peaks
- ② Overload from too much sample
- ③ Temperature drift shifts retention
- ④ Contaminated mobile phase adds noise
- ⑤ Mismatched standards bias MW
❓ FAQ
+Is GPC absolute?
Usually relative; use light scattering for absolute.
+What if dispersity is large?
Broad distribution; check polymerization control.
+Switch mobile phase?
Use compatible columns and re-calibrate.