📋 Workflow
Full workflow, products add-to-cart directly1
Carrier gas & column install
20 minInstall the capillary column to the detector and purge carrier gas for leak check
💡 Tip Do not overtighten column nuts
⚠️ Caution Ensure carrier gas purity before use
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2
Standard & sample prep
30 minDilute standards and samples with solvent; derivative if needed
💡 Tip Internal standard quantitation is more robust
⚠️ Caution Store volatile samples at low temperature
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3
Temperature program
20 minSet initial temperature, ramp rate and final temperature to achieve separation
💡 Tip Gradual ramping improves peak shape and resolution
⚠️ Caution Do not exceed column max temperature
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4
Injection & detection
30 minInject via micro-syringe or headspace; detect with FID or MS
💡 Tip Headspace suits residual solvent analysis
⚠️ Caution Avoid air bubbles in the syringe
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5
Quant & data
30 minIntegrate peak areas and calculate concentration by calibration or internal standard
💡 Tip Use internal standard to correct injection errors
⚠️ Caution Run system suitability tests regularly
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Septum bleed causing ghost peaks
- ② Dirty liner causing adsorption/tailing
- ③ Fast ramp reducing resolution
- ④ Volatile loss lowering quantitation
- ⑤ Excess injection causing overload
❓ FAQ
+What samples suit GC?
Vaporizable, low-boiling, thermally stable compounds.
+FID or MS?
Use FID for routine quantitation; MS for identification/high sensitivity.
+Why peak tailing?
Usually liner/column inlet contamination or active site adsorption.