📋 Workflow
Full workflow, products add-to-cart directly1
Wavelength & calibration
20 minWarm up the lamp; calibrate wavelength and intensity with Raman/Rayleigh and a standard
💡 Tip Calibrate sensitivity with a fluorescence standard
⚠️ Caution Block ambient light from the optical path
🧪 Materials for this step (click to shop by spec/brand)
2
Standard prep
20 minPrepare a series of fluorescent standards and measure intensity
💡 Tip Low concentrations behave linearly and sensitively
⚠️ Caution High concentration causes quenching
🧪 Materials for this step (click to shop by spec/brand)
3
Sample & blank reading
20 minMeasure blank then sample fluorescence intensity and subtract the blank
💡 Tip Keep temperature stable as it affects intensity
⚠️ Caution Bubbles scatter and interfere
🧪 Materials for this step (click to shop by spec/brand)
4
Quant & data
20 minConstruct a calibration curve and calculate sample concentration
💡 Tip Express intensity in relative fluorescence units
⚠️ Caution Dilute and re-measure if beyond linear range
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① High concentration causing quenching
- ② Contaminated blank raising background
- ③ Temperature fluctuation causing drift
- ④ Scratched cuvette causing scatter
- ⑤ Aging lamp uncalibrated affecting reproducibility
❓ FAQ
+How more sensitive is fluorescence?
Usually 2–3 orders of magnitude more sensitive.
+How to measure non-fluorescent?
Derivatize or label with a fluorescent probe.
+Why fluctuating intensity?
Usually temperature, lamp or cuvette contamination.