📋 Workflow
Full workflow, products add-to-cart directly1
Seed culture
1 dayActivate strain from glycerol stock/plate, expand in broth to log phase as seed
💡 Tip Seed viability and purity are critical
⚠️ Caution Keep consistency across batches
🧪 Materials for this step (click to shop by spec/brand)
2
Reactor loading and sterilization
2 hLoad fermentation medium into reactor, sterilize in-place and cool to inoculation temperature
💡 Tip Calibrate pH/DO electrodes
⚠️ Caution Sterilize thoroughly to prevent contamination
🧪 Materials for this step (click to shop by spec/brand)
3
Inoculation and culture
1–3 daysInoculate seed, control temperature, pH, dissolved oxygen and agitation, monitor and feed
💡 Tip Feeding strategy optimizes yield
⚠️ Caution Low dissolved oxygen suppresses growth
🧪 Materials for this step (click to shop by spec/brand)
4
Harvest and analysis
2 hHarvest biomass or broth, measure product titer, biomass and purity
💡 Tip Sampling frequency follows process phase
⚠️ Caution Time harvest to product peak
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete sterilization causes contamination
- ② Uncontrolled DO/pH suppresses growth
- ③ Poor feeding lowers yield
- ④ Uncalibrated electrodes cause control drift
- ⑤ Contaminated/aged seed fails batch
❓ FAQ
+Notes for fermentation scale-up?
Keep DO, mixing and shear consistent; scale up stepwise via pilot.
+How to prevent contamination?
Strict sterilization, sealing, aseptic handling and monitoring counts/cleanliness.
+Fed-batch vs batch?
Fed-batch continuously supplies nutrients to avoid substrate inhibition, higher yield but more complex.