📋 Workflow
Full workflow, products add-to-cart directly1
Genomic DNA extraction and QC
2 hExtract gDNA, check concentration, purity and integrity
💡 Tip High-quality DNA ensures complete conversion
⚠️ Caution Degraded/contaminated DNA affects results
🧪 Materials for this step (click to shop by spec/brand)
2
Bisulfite conversion
3–4 hTreat DNA with bisulfite to deaminate unmethylated C to U, purify
💡 Tip Conversion efficiency is critical
⚠️ Caution Control conditions to avoid degradation
🧪 Materials for this step (click to shop by spec/brand)
3
PCR amplification and detection
2–3 hDesign methylation-specific primers for PCR, or use BSP sequencing/quantitative detection
💡 Tip Primers distinguish methylated/unmethylated
⚠️ Caution Include methylated/unmethylated controls
🧪 Materials for this step (click to shop by spec/brand)
4
Data analysis
1 hCalculate methylation level against standards, or map each CpG state by sequencing
💡 Tip Integrate multiple CpG sites
⚠️ Caution Genome-wide needs dedicated methods
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Incomplete conversion gives false methylation
- ② DNA degradation fails conversion/amplification
- ③ Non-specific primers amplify background
- ④ Missing controls hamper quantification
- ⑤ Over-deamination loses methylation
❓ FAQ
+What is MSP?
PCR with primers distinguishing methylated vs unmethylated sequences, simple qualitative detection.
+How to assess conversion efficiency?
Use known non-CpG cytosines as internal controls; all should convert to T.
+Genome-wide methylation sequencing?
Use WGBS or methylation arrays for genome-wide coverage.