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♨️ Sample Prep · Method

digestion

Decompose matrix
🎯 Difficulty ★★☆ Medium Duration About 2–4 h 🎯 Use Decompose organic/complex samples into clear solution for elemental determination
📖 Principle
Acids and oxidants decompose organics under heat/pressure to bring elements into solution
Below are 5 steps. Open each to see how to do it and add products to your shared list.

📋 Workflow

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1

Weigh & pretreat

15 min
Weigh an appropriate sample amount and homogenize or dry it
💡 Tip Record wet weight and moisture
⚠️ Caution Match sample amount to reagent capacity
🧪 Materials for this step (click to shop by spec/brand)
2

Add digestion reagents

10 min
Add nitric acid and oxidant; seal the digestion vessel
💡 Tip HNO₃–H₂O₂ is common
⚠️ Caution Add acid in fume hood
🧪 Materials for this step (click to shop by spec/brand)
3

Microwave/wet digestion

60 min
Heat to target temperature/pressure per program to complete digestion
💡 Tip Stepwise heating avoids bursting
⚠️ Caution Monitor temperature/pressure alarms
🧪 Materials for this step (click to shop by spec/brand)
4

Cool & make up

30 min
Cool, transfer the digest and make up to volume in a volumetric flask
💡 Tip Rinse vessel walls with ultrapure water
⚠️ Caution Cool fully to avoid error
🧪 Materials for this step (click to shop by spec/brand)
5

Blank & QC

20 min
Digest a blank in parallel and include QC to verify recovery
💡 Tip Blank assesses reagent-introduced elements
⚠️ Caution At least one blank per batch
🧪 Materials for this step (click to shop by spec/brand)

⚠️ 5 Common Beginner Mistakes

  • Incomplete digestion lowering results
  • Excessive temperature causing burst/volatilization
  • Reagent blank contamination raising background
  • Incomplete cooling affecting make-up
  • Evaporation to dryness losing analytes

❓ FAQ

+Microwave or wet digestion?
Microwave is faster/safer; wet is cheaper for routine.
+Which acids to digest?
Nitric acid primarily, optionally with H₂O₂ or HCl.
+Why a blank?
Subtract reagent-introduced background.

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Data authenticity: Product categories from JW Supply Chain standard taxonomy; method→step→product connected. Last updated: 2026-08-23 · v3.0