📋 Workflow
Full workflow, products add-to-cart directly1
Single-cell dissociation
30 minGently dissociate pluripotent cells to single cells and plate at density to start differentiation
💡 Tip Add ROCK inhibitor to improve survival
⚠️ Caution Even single-cell distribution
🧪 Materials for this step (click to shop by spec/brand)
2
Stage-wise factor treatment
1–4 weeksAdd Wnt activation/inhibition, FGF and small molecules in sequence to specify the lineage
💡 Tip Optimize concentration/timing per stage
⚠️ Caution Wrong timing fails or derails differentiation
🧪 Materials for this step (click to shop by spec/brand)
3
Differentiation detection
1–2 daysVerify identity and purity by immunofluorescence or qPCR of lineage-specific markers
💡 Tip Cross-confirm with multiple markers
⚠️ Caution Assess contaminating/undifferentiated cells
🧪 Materials for this step (click to shop by spec/brand)
4
Maturation and purification
1–2 weeksExtend culture for maturation, enrich target cells by FACS or magnetic beads
💡 Tip Maturation time varies by cell type
⚠️ Caution Maturation needs proper nutrients/signals
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong factor timing derails direction
- ② Improper density/matrix lowers efficiency
- ③ Insufficient maturation reduces function
- ④ Residual undifferentiated cells pose tumor risk
- ⑤ Batch-to-batch variability in efficiency
❓ FAQ
+How to improve differentiation efficiency?
Optimize stage factor concentration/timing, dissociation, matrix and maturation.
+How to verify success?
Use lineage markers by IF/qPCR, plus morphology and functional endpoints.
+High cell death during differentiation?
Optimize factors/feeding, add survival factors or ROCK inhibitor, avoid overtight passaging.