📋 Workflow
Full workflow, products add-to-cart directly1
Cell preparation
30 minHarvest log-phase cells, count ensuring high viability and good condition
💡 Tip Cryopreserve only healthy low-passage cells
⚠️ Caution Digest gently to avoid damage
🧪 Materials for this step (click to shop by spec/brand)
2
Prepare cryomedium
10 minResuspend cells in freezing medium with 5-10% DMSO final
💡 Tip Pre-cool medium reduces toxicity
⚠️ Caution Prolonged DMSO exposure at RT harms cells
🧪 Materials for this step (click to shop by spec/brand)
3
Aliquot and cool
1 hAliquot into cryovials, cool ~1°C/min in controlled-rate box or -80°C, then liquid nitrogen
💡 Tip Gradient cooling prevents ice crystals
⚠️ Caution Label strain, passage and date
🧪 Materials for this step (click to shop by spec/brand)
4
Thawing
30 minThaw rapidly in 37°C bath, transfer to pre-warmed medium, centrifuge out DMSO and seed
💡 Tip Rapid thaw minimizes osmotic injury
⚠️ Caution Handle gently to prevent rupture
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Poor cell state before freezing causes high death on recovery
- ② Too-fast cooling forms ice crystals damaging cells
- ③ Incomplete DMSO removal causes toxicity
- ④ Uncooled cryomedium worsens DMSO osmotic injury
- ⑤ Missing labels cause strain confusion
❓ FAQ
+How long until usable after recovery?
Usually 1-2 passages to recover; passage once before experiments.
+How to assess cryopreservation?
Check viability by trypan blue or flow within 24 h, >80% is good.
+How many cells per vial?
Typically 1-2×10^6/vial; more for clones or rare cells.