📋 Workflow
Full workflow, products add-to-cart directly1
Target cell preparation
1 hCulture and label target cells, count and resuspend at suitable density
💡 Tip Load dye/radio label to detect lysis
⚠️ Caution High viability ensures reliable results
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2
Antibody sensitization
30 minIncubate targets with excess antibody to sensitize them for complement activation
💡 Tip Excess antibody ensures saturation
⚠️ Caution Wash off free antibody to control background
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3
Complement treatment
1 hAdd complement source (human/rabbit serum) and incubate to activate lysis
💡 Tip Control complement titer/stability
⚠️ Caution Keep complement cold to prevent inactivation
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4
Lysis detection
1 hMeasure released marker in supernatant or stain live cells to calculate lysis percentage
💡 Tip Include spontaneous and maximum release controls
⚠️ Caution High background masks differences
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Inactivated complement gives false negatives
- ② High spontaneous release masks complement activity
- ③ Insufficient antibody reduces lysis
- ④ Excess complement causes non-specific lysis
- ⑤ Missing maximum release hampers lysis calculation
❓ FAQ
+Which controls are necessary?
Spontaneous (no complement), maximum (lysis agent) and no-antibody controls are essential.
+How to improve complement lysis?
Optimize antibody density, complement titer, target sensitivity and matched source.
+How to store active serum?
Use fresh or aliquot at -80°C, avoiding repeated freeze-thaw and heat inactivation.