📋 Workflow
Full workflow, products add-to-cart directly1
T-cell isolation and activation
1–2 daysIsolate T cells from PBMC and activate with anti-CD3/CD28 beads
💡 Tip Activators and IL-2 support expansion
⚠️ Caution Aseptic handling prevents contamination
🧪 Materials for this step (click to shop by spec/brand)
2
CAR gene transduction
1 dayTransduce activated T cells with CAR expression vector by lentivirus or electroporation
💡 Tip Optimize MOI and transduction conditions
⚠️ Caution Recover cells from transduction stress
🧪 Materials for this step (click to shop by spec/brand)
3
Expansion and culture
1–2 weeksExpand CAR-T cells to target numbers in medium with cytokines like IL-2
💡 Tip Monitor expansion and viability
⚠️ Caution Control density and nutrients
🧪 Materials for this step (click to shop by spec/brand)
4
Quality check and infusion
1 dayCheck CAR expression, fold expansion and killing activity; wash and infuse if criteria met
💡 Tip Quantify CAR by FACS
⚠️ Caution Bacterial/endotoxin QC before infusion
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Poor T-cell source limits expansion
- ② Low transduction limits CAR expression
- ③ Over-culture exhausts T cells
- ④ Contamination or QC failure
- ⑤ Insufficient killing validation affects efficacy
❓ FAQ
+How to choose CAR-T target?
Choose antigens highly expressed on tumor but low on normal tissue (CD19, BCMA) to limit off-target toxicity.
+How to avoid T-cell exhaustion?
Limit expansion time/density, optimize cytokine cocktail, preserve early memory phenotype.
+Autologous vs allogeneic CAR-T?
Autologous is individualized but slow; allogeneic is off-the-shelf but needs immunosuppression and GVHD control.