📋 Workflow
Full workflow, products add-to-cart directly1
Prepare standard curve
15 minDilute BSA standard into a gradient to build a concentration-absorbance calibration curve
💡 Tip Standards quality determines accuracy
⚠️ Caution Match sample buffer system
🧪 Materials for this step (click to shop by spec/brand)
2
Prepare BCA working reagent
10 minMix reagent A and B at ratio, store at RT in dark
💡 Tip Prepare fresh to avoid degradation
⚠️ Caution Protect from light for even color
🧪 Materials for this step (click to shop by spec/brand)
3
Dispense and incubate
30 minMix samples with working reagent, incubate 30 min at 37°C for color development
💡 Tip Dilute concentrated samples and factor in dilution
⚠️ Caution Reducing agents interfere with color development
🧪 Materials for this step (click to shop by spec/brand)
4
Read and calculate
10 minRead at 562 nm on plate reader, calculate concentrations from the standard curve
💡 Tip Samples fall in linear range
⚠️ Caution Subtract blank background
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Reducing agents (DTT) interfere
- ② High detergent concentration interferes
- ③ Buffer mismatch between standards and samples
- ④ Sample beyond linear range
- ⑤ Light-degraded reagent gives uneven color
❓ FAQ
+BCA vs Bradford?
BCA tolerates most detergents and is robust; Bradford is faster but detergent-sensitive.
+How to measure with reducing agents?
Switch to Bradford (reducing-agent compatible) or remove/dilute the reducing agent.
+Precision across concentration range?
Dilute high, load more for low, stay in mid-linear range for accuracy.