📋 Workflow
Full workflow, products add-to-cart directly1
Triple-plasmid co-transfection
1 dayCo-transfect cargo, rep/cap and helper plasmids into high-density 293T cells
💡 Tip Optimize plasmid ratios
⚠️ Caution Transfection efficiency determines titer
🧪 Materials for this step (click to shop by spec/brand)
2
Culture and viral harvest
3–5 daysCulture several days, harvest supernatant and/or lyse cells to release virus
💡 Tip AAV harvest both intra- and extracellular
⚠️ Caution Harvest timing affects titer
🧪 Materials for this step (click to shop by spec/brand)
3
Purification and concentration
1 dayPurify and concentrate viral particles by ultracentrifugation, affinity column or PEG precipitation
💡 Tip Purification improves purity and activity
⚠️ Caution Avoid shear force inactivating virus
🧪 Materials for this step (click to shop by spec/brand)
4
Titer determination and QC
1 dayMeasure genome titer by qPCR or infectious titer by GFP/infection, run endotoxin QC
💡 Tip Measure both genome and infectious titer
⚠️ Caution Avoid cross-contamination and false titer
🧪 Materials for this step (click to shop by spec/brand)
⚠️ 5 Common Beginner Mistakes
- ① Wrong plasmid ratio lowers titer
- ② Poor 293T state fails packaging
- ③ Shear/freeze-thaw inactivates virus
- ④ Incomplete purification contaminates
- ⑤ Titer error misjudges dosage
❓ FAQ
+AAV vs lentivirus?
AAV non-integrating, low immunogenicity for in vivo; lentivirus integrates for stable expression.
+How to boost titer?
Optimize plasmid ratio, transfection efficiency, harvest timing and purification.
+Why two titer types?
Genome titer = total particles; infectious titer = effective entry; compare for quality.