Genovis offers affinity reagents to detect residual enzyme levels in complex samples. Goat polyclonal antibodies detecting the enzymes are available for the FabRICATOR (IdeS), FabRICATOR Z (IdeZ) and Xork enzymes. The antibodies can be used to detect the enzymes in Western blot and ELISA assays, as well as in preclinical gene therapy applications.

 The antibodies are qualified for the use of e.g., western blot and ELISA to detect the FabRICATOR Z enzyme. Here, we show the performance of both Anti-FabRICATOR Z Affinity Purified as well as Anti-FabRICATOR Z Affinity Purified Biotin conjugated.

 The wells were coated with Anti-FabRICATOR Z Affinity Purified at 2 μg/ml. The FabRICATOR Z enzyme was added in dilutions from 2 μg/ml to 2 ng/ml. Anti-FabRICATOR Z Affinity Purified Biotin Conjugated was added at 1 μg/ml. HRP-Streptavidin was added in a 1:2000 dilution.