Immobilized enzymes for desialylation of glycoproteins in spin columns.

 The SialEXO Immobilized spin columns contain SialEXO enzyme covalently coupled to agarose beads, for desialylation of glycoproteins without contaminating the final preparation with enzyme. It hydrolyzes sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins and released glycans.

 The Microspin columns are available for desialylation of 2 x 0.5 mg, 5 x 0.5 mg, or 10 x 0.5 mg glycoprotein.

•Easy-to-use spin columns

•Increased enzyme-to-substrate ratio for improved digestion efficiency

•No enzyme in the final sample