Site-specific conjugation of IgG with DFO.

 GlyCLICK is a site-specific conjugation technology for IgG using Fc glycan remodeling and click-chemistry. The technology generates stable and homogenous antibody conjugates for IgG from several species and subclasses. Fc-glycan remodeling by complete deglycosylation of the antibody allows for site-specific conjugation using robust click-chemistry, resulting in a degree of label (DOL) or drug-antibody ratio (DAR) of 2.

 GlyCLICK DFO generates conjugates carrying deferoxamine (DFO), a chelator used for metal-tagging or radiolabeling of IgG for in vivo and in vitro imaging. In contrast to randomly conjugated antibody PET-tracers, site-specific labeling at the Fc glycan sites using GlyCLICK DFO generates tracers that exhibit increased target-affinity, improved stability and a lower off-target accumulation.

 GlyCLICK DFO is available for site-specific labeling of 250 μg or 2 mg IgG with the metal chelator DFO (deferoxamine), and is suitable for in vivo and in vitro radiolabeling and imaging.

•Site-specific DFO-conjugation to IgG with a degree of labeling (DOL) of 2

•Facilitates radiolabeling for PET/SPECT imaging

•Improves in vivo imaging quality