Corning® Fibronectin, human, 5mg, is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.

Quality

Purity = 90% by SDS-PAGE

Tested for ability to promote attachment and spreading of BHK-21 cells

Found negative for bacteria, fungi, and mycoplasma

Source

Human plasma. Source material tested for hepatitis B antigen and HIV-1 antibody.

Molecular Weight

440 kD (unreduced form)

Preparation and Storage

Stable for three months at 2° to 8°C (lyophilized) or two weeks at -20°C (solubilized)

Handling

Recommended concentration - 1-5 µg/cm² of growth surface for coating or at 5 µg/ml as a media additive

Formulation

Lyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0